If we want to understand a biological organism, we turn to the expression of its genome. Which genes are being expressed, and in which cells, and when? How does this differ between a normal cell and a cancer cell? We have incredibly sophisticated techniques to investigate these questions, and the most indispensable is the DNA microarray assay. This apparatus is even more useful than it is colorful, so let’s see how this works now.
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Gene Expression Analysis and DNA Microarray Assays
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Gene Expression Analysis and DNA Microarray Assays
cdna technology
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22 comments
this man is a legend i have an assignment due tmrow that if i fail im gonna have a 70 and this man just saved me
I'm doing a biochemistry masters and this is helping me – thanks.
@5:26 Do I have this right? (no background in science, just leaping in)
-There's a double stranded cDNA: one is identical to the DNA template and the other is complementary to template
–These two strands are bound together, right?
-"One of the two strands of some cDNA molecule made from an mRNA ought to bind to the DNA fragment in a particular well"
–How does one of the strand bind to the DNA fragment? My assumption is that the cDNA are bound to each other, so how does one of them separate and bind to the fragment?
I have a question here.. Do we amplify cDNA before introducing it to the Array? If yes, we already used a primer to amplify our gene of interest.. In this case, how different genes in the array well would produce color except the one we amplified with our primer? If we don't amplify, how the trace amount of different cDNAs will produce color in array?
Watching this to clarify my mind on my thesis proposal tomorrow, you're a life saver! THX
What kind of sequencing can you perform that Dave explains at 7:15? can you do Next gen sequencing?
Thank you please more things explain about genotype
Wow, nice video man
I FREAKING LOVE YOU PROFESSOR DAVE EXPLAINS!!!!! <3
Genius
Thank you so much !
My question is mRNA already bound and complementary cDNA is formed then how they bind with dna probes present in wells
Many thanks for this explanation professor Dave!
Thank you Professor Dave 😃
med student here. wish our professors can teach like this…
Sir but how can this be used for quantitative assay of a particular gene…i mean i only understand that this will be only useful for qualitative analysis
YOU ARE THE BEST
I thought this was some advanced connect4 technique.
Microarrays in 2020? Omegalul
thank you for your content. you explained it beautifully
Amazing !
thank you sir