Gene Expression Analysis and DNA Microarray Assays cdna technology

by alsaCEMusic



If we want to understand a biological organism, we turn to the expression of its genome. Which genes are being expressed, and in which cells, and when? How does this differ between a normal cell and a cancer cell? We have incredibly sophisticated techniques to investigate these questions, and the most indispensable is the DNA microarray assay. This apparatus is even more useful than it is colorful, so let’s see how this works now.

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Gene Expression Analysis and DNA Microarray Assays

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Gene Expression Analysis and DNA Microarray Assays
cdna technology
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22 comments

maylia 313 20/01/2022 - 4:10 Chiều

this man is a legend i have an assignment due tmrow that if i fail im gonna have a 70 and this man just saved me

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Max R 20/01/2022 - 4:10 Chiều

I'm doing a biochemistry masters and this is helping me – thanks.

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Philo-Sophy 20/01/2022 - 4:10 Chiều

@5:26 Do I have this right? (no background in science, just leaping in)
-There's a double stranded cDNA: one is identical to the DNA template and the other is complementary to template
–These two strands are bound together, right?
-"One of the two strands of some cDNA molecule made from an mRNA ought to bind to the DNA fragment in a particular well"
–How does one of the strand bind to the DNA fragment? My assumption is that the cDNA are bound to each other, so how does one of them separate and bind to the fragment?

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Polymath 20/01/2022 - 4:10 Chiều

I have a question here.. Do we amplify cDNA before introducing it to the Array? If yes, we already used a primer to amplify our gene of interest.. In this case, how different genes in the array well would produce color except the one we amplified with our primer? If we don't amplify, how the trace amount of different cDNAs will produce color in array?

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Victoria Ríos Vázquez 20/01/2022 - 4:10 Chiều

Watching this to clarify my mind on my thesis proposal tomorrow, you're a life saver! THX

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Matt 20/01/2022 - 4:10 Chiều

What kind of sequencing can you perform that Dave explains at 7:15? can you do Next gen sequencing?

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sofian bio 20/01/2022 - 4:10 Chiều

Thank you please more things explain about genotype

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Ismael Elhasadi 20/01/2022 - 4:10 Chiều

Wow, nice video man

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Omar Alblooshi 20/01/2022 - 4:10 Chiều

I FREAKING LOVE YOU PROFESSOR DAVE EXPLAINS!!!!! <3

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Adnan Qamar 20/01/2022 - 4:10 Chiều

Genius

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Sushma Nepal 20/01/2022 - 4:10 Chiều

Thank you so much !

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MUHAMMAD ANEES 20/01/2022 - 4:10 Chiều

My question is mRNA already bound and complementary cDNA is formed then how they bind with dna probes present in wells

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BracKen 20/01/2022 - 4:10 Chiều

Many thanks for this explanation professor Dave!

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Eman Nadeem 20/01/2022 - 4:10 Chiều

Thank you Professor Dave 😃

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Jonathan Kim 20/01/2022 - 4:10 Chiều

med student here. wish our professors can teach like this…

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sri ram 20/01/2022 - 4:10 Chiều

Sir but how can this be used for quantitative assay of a particular gene…i mean i only understand that this will be only useful for qualitative analysis

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MA. 20/01/2022 - 4:10 Chiều

YOU ARE THE BEST

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shan Zhu 20/01/2022 - 4:10 Chiều

I thought this was some advanced connect4 technique.

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David Wang 20/01/2022 - 4:10 Chiều

Microarrays in 2020? Omegalul

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wolverine 20/01/2022 - 4:10 Chiều

thank you for your content. you explained it beautifully

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Dr Shreya agrawal 20/01/2022 - 4:10 Chiều

Amazing !

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Smit Shah 20/01/2022 - 4:10 Chiều

thank you sir

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