DNA Libraries | Genomic and cDNA Library | cdna technology

by alsaCEMusic



scientists created DNA libraries, which represent collections of cloned DNA. Depending on how a library is constructed, it may contain genes and noncoding regions of DNA. Generally speaking, there are two main types of libraries, genomic DNA libraries and complementary DNA (cDNA) libraries.
Genomic Libraries
Ideally, a genomic library consists of many overlapping fragments of the genome, with at least one copy of every DNA sequence in an organism’s chromosomes, which in summary span the entire genome.
In making a genomic library, chromosomal DNA is extracted from cells or tissues and cut randomly with restriction enzymes, and the resulting fragments are inserted into vectors.
Complementary DNA (cDNA) Libraries
Complementary DNA (cDNA) libraries offer certain advantages over genomic libraries and continue to be a useful methodology for specific approaches to gene cloning and other applications. This is primarily because a cDNA library contains DNA copies—called cDNA—which are made from mRNA molecules isolated from cultured cells or a tissue sample. A cDNA library therefore represents only the genes being expressed in cells at the time the library was made—unlike a genomic library, which contains all of the DNA, coding and noncoding, in a genome.
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36 comments

Sohrab Khan 29/12/2021 - 5:31 Chiều

Sir ap Kon se board se parahte ho

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Fazal Ur Rehman 29/12/2021 - 5:31 Chiều

Brother Aslam o Alaikum…!!
Please give a video lecture on Genomics, Transcriptomics, Proteomics and Metablomics.

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Manoj Naharwal 29/12/2021 - 5:31 Chiều

Sir I have doubt . Dna molecule ke sath to dna molecule hi attach hote h RNA thodi hote h attach

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Adeel Mehmood 29/12/2021 - 5:31 Chiều

Sir, make video on pkd/adpkd diseases which Crispr-cas9 protocol. There are two genes called PKD1, PKD2 by mutations thanks

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BHATTI SHAH 29/12/2021 - 5:31 Chiều

Nice lectures sir but plz also tell the definitions of topic and some key points in the board overall thanks for best delivering leactures

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Omeshka NaeemKhan 29/12/2021 - 5:31 Chiều

All your lectures are Amazing 💜

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bhavya bhardwaj 29/12/2021 - 5:31 Chiều

your lecture ,your given notes is very useful for me , every time …. 🙏 thankyou sir.. for making such a type of useful video for us

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Rajvi Patel 29/12/2021 - 5:31 Chiều

Thanks for making preparations easier at exam time

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Rizwan Khan 29/12/2021 - 5:31 Chiều

Very useful video, thank you so much 🥰❣️

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soman pk 29/12/2021 - 5:31 Chiều

Thank you

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shujat ali 29/12/2021 - 5:31 Chiều

Appreciated

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priyanka jha 29/12/2021 - 5:31 Chiều

❤️

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priyanka jha 29/12/2021 - 5:31 Chiều

Wow 🤗♥️💐🙏🌸✌️ splendid ❤️💙💓❤️💜💓💙

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MDC 29/12/2021 - 5:31 Chiều

Sir pehle bar aj probe ka kaam samj aaya thank u

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Swati Saini 29/12/2021 - 5:31 Chiều

Very helpful lecture

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mystic dust 29/12/2021 - 5:31 Chiều

Amazing lecture, a lots of love from india

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Aaru 1234 1234nadaf 29/12/2021 - 5:31 Chiều

Waliekumasalaam
Thank you soo much sir

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Puja Dey 29/12/2021 - 5:31 Chiều

Thank you so much…

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Owais hassan Khan 29/12/2021 - 5:31 Chiều

Assalam alakum sir, Now i am in 2ñd yr but i have already watched your first yr lectures all and give pray to you on every topics but sir there are some chapter that are not uploaded for example.
(1) Diversity among Animals.
(2)Form and function of plant.
(3) Diversity among plant.
Other wise love you sir from swat. Your the world best teacher.i love your simplicity sir.

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Ashfaq Peerzade 29/12/2021 - 5:31 Chiều

Aap nahi jante Aap mere liye kya ho ❤️

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Shahrukh 29/12/2021 - 5:31 Chiều

MashAllah💖😍

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Shajia Khan 29/12/2021 - 5:31 Chiều

Sir your videos are really very good.Your videos are always beneficial for me.Thank you very much sir👍👍👍👍👏👏👏👏🤲🤲🤲🤲

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The Calculated Food 29/12/2021 - 5:31 Chiều

Sir hum ny tu already ik fragment ly k plasmid mai insert kiya tha…. Usi fragment ko hum ny host cell k through replicate krwaya means same fragment of DNA so again exraction hm ny q ki?

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Ashok garg 29/12/2021 - 5:31 Chiều

Sir aap ne mbbs कि हुई है kya

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Shivani Baliyan 29/12/2021 - 5:31 Chiều

Thanku so much sir.. It's really very helpful 😇🙏🙏🙏🙏🙏

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rittumaildesk 29/12/2021 - 5:31 Chiều

I would have left this topic if i have not watched your video….easy n best explanation..thanx

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tanveer rafique 29/12/2021 - 5:31 Chiều

How someone can be sooooooo good😩😩😩😩😩😩❤️❤️❤️❤️ teacher

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Gurjeevan Raj 29/12/2021 - 5:31 Chiều

😍 Awesome 😍

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Shanu Pandey 29/12/2021 - 5:31 Chiều

Sir make a video on molecular markers ….will really help✌

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Deepak Thakur 29/12/2021 - 5:31 Chiều

You're great brother 💯thank you so much for your efforts for students

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ABHIPSA MISHRA 29/12/2021 - 5:31 Chiều

Very nice lecture…

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light 29/12/2021 - 5:31 Chiều

Superb

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Palwasha Meherr 29/12/2021 - 5:31 Chiều

Plz share ur journey of youtuber and reach to 200k subscriber

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Palwasha Meherr 29/12/2021 - 5:31 Chiều

♥️One of the best teacher

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Aditi Singh 29/12/2021 - 5:31 Chiều

Outstanding 👍👍👌👌❤️

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Ijaz Ahmad 29/12/2021 - 5:31 Chiều

good job👍

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